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+ Resize images and save to another location.. Connect your Music Player/Cloud Player and let . this study can be useful for future works on synthesizing peptides that mimic a variety of amino acid sequences, for example, silk peptides derived from silk worms and silk proteins derived from *Bombyx mori*.

4. Conclusions {#sec4-materials-10-00880}
==============

This study has demonstrated the usefulness of *N*-terminal and *C*-terminal amino acid sequences of the sericin protein to synthesize silk peptides using an Fmoc chemistry-based SPPS method. The predicted secondary structure of each synthesized peptide was confirmed and thus proved to be a useful tool for prediction. This study also confirmed the feasibility of synthesizing a silk peptide containing all amino acids (alanine, threonine, and serine) of the seed region of sericin I1. This synthetic silk peptide might be developed as a novel silk material with functionalities that mimic silk fibers.

Part of this work was supported by the Japan Society for the Promotion of Science (JSPS) through JSPS KAKENHI Grant Numbers JP25220602, JP15K14569, and JP16H05517.

T.Y. designed the experiments, synthesized all peptides, and wrote the paper; K.F., T.Y., and S.S. performed the experiments and analyzed the data; K.M. and S.Y. designed the experimental setup; N.S. and R.S. conceived and supervised this work and reviewed the manuscript.

The authors declare no conflict of interest.

![Schematic representation of silk synthesis by *N*-terminal and *C*-terminal sequence analysis. (**A**) Schematic representation of the purified silk protein. Sericin protein contains *N*-terminal (Ser-1 to 3) and *C*-terminal (Ser-104 to 105) regions (Black filled bar). And the silk protein contains 23 amino acids from Pro-

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